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#1
02-20-2004, 10:16 PM
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 looking for good antibodies for any tag Hi there! I am expressing an unstable protein tagged with 6His (and its mutants) in protoplasts and trying to detect it using anti-6His monoclonal antibodies from Novagen by western blotting. My results are far from acceptable at this stage despite the ~ 50-60% transformation efficiency I get each time I transfect protoplasts. Could anyone give me some advice on great sources of antibodies for small tags (6His, HA, myc,...)? Thank you so much in advance! Sincerely, Miguel ---  |
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| antibodies , good , tag |
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