Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Arabidopsis and Plant Biology
Register Search Today's Posts Mark Forums Read

Arabidopsis and Plant Biology Discuss Arabidopsis and Plant Biology Research.

Agrobacterium transformation

Agrobacterium transformation - Arabidopsis and Plant Biology

Agrobacterium transformation - Discuss Arabidopsis and Plant Biology Research.

LinkBack Thread Tools Display Modes
Old 01-05-2004, 05:40 PM
Zhixiang Chen
Posts: n/a
Default Agrobacterium transformation

Dear Colleagues:

We are trying to use the series of binary vectors (pAOV, pKMB, pSOV etc.)
developed by Joshua Mylne in Australia (Plant Molecular Biology 1998,
16:257-262) for constitutive expression of Arabidopsis genes and are having
problem transforming them into the GV3101 strain of agrobacterium. These
vectors were constructed using pSLJ75515 as backbone and are supposed to
have tet resistance in both E. coli and agrobacterium. We have tried direct
transformation (using competent agrobacterial cells) and tri-parental mating
but couldn't get any positive transformant. We would greatly appreciate it
if someone there who has used these vectors or the related pSLJ75515 could
share with us the detailed protocol of transforming these vectors into
GV3101 (including the medium and antibiotic used,their concentrations and
culture conditions, length etc.). Thank you very much for your help.

Zhixiang Chen, Associate Professor
Department of Botany & Plant Pathology
915 West State Street
Purdue University
West Lafayette, IN 47907-2054

Phone: 765-494-4657
FAX: 765-494-5896
E-mail: [Only registered users see links. ]

Reply With Quote

agrobacterium , transformation

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
pENTR1A Transformation not working CambridgeBiochemist Molecular Cloning Forum 1 06-18-2012 06:53 AM
Regarding your question on Agrobacterium transformation shahzad anjam Arabidopsis and Plant Biology 0 11-03-2009 04:31 PM
Agrobacterium Transformation and Over-growth? moleculardude Arabidopsis and Plant Biology 0 12-03-2008 02:20 PM
What is the different between the plasmid that used to overexpressprotein in E coli and that for Agrobacterium to transformation plants? guo shukui Arabidopsis and Plant Biology 0 12-01-2006 05:33 PM
Agrobacterium strains Sheshagiri R Protocols and Methods Forum 2 06-09-2006 01:46 AM

All times are GMT. The time now is 05:41 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11043 seconds with 16 queries