Nuclear protein extraction for plants

[Genesys]

Hi everybody,

Im looking for a nuclear protein extraction protocol for myplant: Lotus japonicus (leguminosae). I saw in litterature that the chemichals were always changing from plant to plant (kind of chemichals and their respective amounts in the extraction buffers). I wonder if somebody of u could help me by providing me the best protocol to extract nucleus from frozen plant tissue and then to extract the proteins from the nuclei.

Thx in advance

§.

[kokos]

Hello,

I am also looking for a nuclear protein called 'portoklaniaris ala pagotaris' which I need to use to also extract the tissue of my plant 'fitologidis epistimonidis proteonukloropoulos'. I don't seem to find it anywhere and I need some guidance to locate it so I can complete my experiment.

Thank you in advance

[bsyu]

sorry, I am also looking for a nuclear protein !

[Plantplanet]

Hi,
In fact I want to try this. I never did this by my self but found two papers. Please have a look and try if you think it serves your purpose.
Please let me know the result after your trails.

1. Microextraction of Nuclear Proteins from Single Maize Embryos
Peter K. Busk and Montserrat Pag`es
Departament de Gen`etica Molecular. Centre d’Investigaci´o i
desenvolupament
C.S.I.C. Jordi Girona 1826
08034 Barcelona, Spain
2. Isolation of Active DNA-Binding Nuclear Proteins From Tomato Galls
Induced by Root-Knot Nematodes
CAROLINA ESCOBAR1,*,**, FABIO ARISTIZÁBAL2,3,*,**, ALFONSO
NAVAS4, FRANCISCA F. DEL CAMPO3 and CARMEN FENOLL1

All the best.
Cheers..

[celina]

Hi
I´ve also been tempted to give the "Microextraction of Nuclear Proteins from Single Maize Embryos" protocol a try, but in the end never did.
If anyone does I'd really like to know if it works.
bye bye

[l.bhutto]

I also need a proper method to extract and quantify nuclear proteins from my wheat plants (leaves) under different drought treatments.

Many Thanks
Bhutto
[Only registered users see links. ]

[sask]

Originally Posted by l.bhutto

I also need a proper method to extract and quantify nuclear proteins from my wheat plants (leaves) under different drought treatments.

Many Thanks
Bhutto
[Only registered users see links. ]

Hi, I a new to the forum and working on similar problem as you. Did you find any method that worked for extraction and quantification of nuclear proteins from wheat leaf.
Hope you would reply back asap.
Thanks a lot

[huotoad]

I have tried the "Microextraction of Nuclear Proteins from Single Maize Embryos". I also asked lots of people for telling me about the protein quality. Most of them said positivly. However, there was no binding for my gel shift assay, even using a TATABOX1 as a probe. I am not sure if the protein is really functional or the binding condition is not good enough.

[Jonathan Brandt]

This is good info guys. Thanks!