Nuclear protein extraction for plants
 

Hi everybody,

Im looking for a nuclear protein extraction protocol for myplant: Lotus japonicus (leguminosae). I saw in litterature that the chemichals were always changing from plant to plant (kind of chemichals and their respective amounts in the extraction buffers). I wonder if somebody of u could help me by providing me the best protocol to extract nucleus from frozen plant tissue and then to extract the proteins from the nuclei.

Thx in advance

§.

Re: Nuclear protein extraction for plants
 

Hello,

I am also looking for a nuclear protein called 'portoklaniaris ala pagotaris' which I need to use to also extract the tissue of my plant 'fitologidis epistimonidis proteonukloropoulos'. I don't seem to find it anywhere and I need some guidance to locate it so I can complete my experiment.

Thank you in advance

Re: Nuclear protein extraction for plants
 

sorry, I am also looking for a nuclear protein !

Re: Nuclear protein extraction for plants
 

Hi,
In fact I want to try this. I never did this by my self but found two papers. Please have a look and try if you think it serves your purpose.
Please let me know the result after your trails.

1. Microextraction of Nuclear Proteins from Single Maize Embryos
Peter K. Busk and Montserrat Pag`es
Departament de Gen`etica Molecular. Centre d’Investigaci´o i
desenvolupament
C.S.I.C. Jordi Girona 1826
08034 Barcelona, Spain
2. Isolation of Active DNA-Binding Nuclear Proteins From Tomato Galls
Induced by Root-Knot Nematodes
CAROLINA ESCOBAR1,*,**, FABIO ARISTIZÁBAL2,3,*,**, ALFONSO
NAVAS4, FRANCISCA F. DEL CAMPO3 and CARMEN FENOLL1

All the best.
Cheers..

Re: Nuclear protein extraction for plants
 

Hi
I´ve also been tempted to give the "Microextraction of Nuclear Proteins from Single Maize Embryos" protocol a try, but in the end never did.
If anyone does I'd really like to know if it works.
bye bye

Re: Nuclear protein extraction for plants
 

I also need a proper method to extract and quantify nuclear proteins from my wheat plants (leaves) under different drought treatments.

Many Thanks
Bhutto
[Only registered and activated users can see links. Click Here To Register...]

Re: Nuclear protein extraction for plants
 

Hi, I a new to the forum and working on similar problem as you. Did you find any method that worked for extraction and quantification of nuclear proteins from wheat leaf.
Hope you would reply back asap.
Thanks a lot

Re: Nuclear protein extraction for plants
 

I have tried the "Microextraction of Nuclear Proteins from Single Maize Embryos". I also asked lots of people for telling me about the protein quality. Most of them said positivly. However, there was no binding for my gel shift assay, even using a TATABOX1 as a probe. I am not sure if the protein is really functional or the binding condition is not good enough.

Re: Nuclear protein extraction for plants
 

This is good info guys. Thanks!