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|Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.|
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What kind of experiment can u conduct to show the relationship between cell death,
cellular senescent & aging? What kind of compound would you use and how would you approach it. A brief explanation would suffice.
Re: What kind of experiment can u conduct to show the relationship between cell death
Your'e asking how to differentiate between whether a cell is dying, undergoing senescence or aging?
-caspase-1 and other caspase genes are good for measuring stress and death and apoptosis. you can do western blots for those genes in different conditions to see what kills them. or you can run qPCR for mRNA level changes in the cells after different stressful conditions.
-detect ROS in the mitochondria and cytoplasm can also tell you about the aging and stress or death levels. you can buy fluorescent probes that bind to free floating oxygen species. checkout MitoSOX Red, or H2DCF-DA probes from invitrogen that you can stain and measure fluorescent intensity levels on a fluorescent microscope
-simple trypan blue for cell viability
-monitoring cell growth either by visual inspection or counting number of cells in a plate. if the cells don't appear to be growing then that's an easy way to see if they are undergoing senescence.
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