Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Apoptosis, Autophagy, and Necrosis Forum
Register Search Today's Posts Mark Forums Read

Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


Differential caspase 3 and 9 mRNA expression

Differential caspase 3 and 9 mRNA expression - Apoptosis, Autophagy, and Necrosis Forum

Differential caspase 3 and 9 mRNA expression - Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-29-2011, 08:26 PM
Pipette Filler
Points: 58, Level: 1 Points: 58, Level: 1 Points: 58, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Differential caspase 3 and 9 mRNA expression



Hello Everybody

After many repeates in real time experiment, I am getting a differential expression of Caspase 3, 9 and P53 genes (no dose response).
Relative to the solvent control a significant 164, 72.4 and 117 % differential up-regulation in the mRNA expression of p53 gene has been observed (Please see attachment Fig). For the caspase 3 and 9 genes, Up-regulation of mRNA expression has been noticed only at two initial doses (0.046, 0.092 mg /kg bw/day). However, the mRNA expression at the highest dose of 0.184 mg /kg bw/day has exhibited a decline in mRNA expression. Relative to control the expression of mRNA for caspase 3 and 9 were 120.7, 202.4, 67.5 and 186, 213, 123.2 % mRNA expression has been observed at 0.046, 0.092 and 0.184 mg /kg bw/day.

In order to confirm the translational product, I have also checked the enzyme activity of Caspase 3 and 9 (no p53). In this experiment 1.2, 1.6-fold and 2.7, 3.6-fold (p<0.05) increase in caspase 3 and 9 activity at 0.092 and 0.184 mg/kg bw/day treated animals has been recoreded.

Query;

As a general rule, if I would get an increase in mRNA expression of Caspase 3 and 9, with low enzyme or protein activity, this suggests a post-translational modification.

However, in my case this is reverse, I am getting the low mRNA expression at the highest dose but the enzyme activity at the same dose is higher.

Therefore, I am stucked at this point and need expert views from the leading workers to resolve my problem (if possible, with a relvant literature to support) which could lead me to draw a meaningful conclusion.

I am attaching the figure of mRNA expression and enzyme activity data for you.


Looking to hearing from you soon.....
Attached Images
File Type: jpg Figure.jpg (85.0 KB, 0 views)
Reply With Quote
Reply

Tags
caspase , differential , expression , mrna


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
qPCR NEWSLETTER - October 2006 Editor www.Gene-Quantification.info Protocols and Methods Forum 0 10-26-2006 12:25 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM


All times are GMT. The time now is 09:56 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.18732 seconds with 17 queries