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Help needed with HL 60

Help needed with HL 60 - Apoptosis, Autophagy, and Necrosis Forum

Help needed with HL 60 - Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


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Old 03-17-2011, 04:21 PM
Pipette Filler
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Default Help needed with HL 60



I am trying to do MTT assay using HL 60 cells .The idea is to testing the activity of natural product on the HL 60.I have been trying to standardise the protocol but no chance,which is:

plate 20,000 cells/well
add extract ,incubate for 3 days
Add 50 ul MTT ,incubate 4 hrs
Centrifuge 3500 g for 10 mins
Discard medium ,add 50 ul DMSO.
Shake 5 mins ,read

Can anyone help me( or improve ) with this protocol

Thanks
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Old 04-19-2011, 02:52 AM
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Default Re: Help needed with HL 60

Which exactly is the problem you're having? Is the cell viability too low even in the negative control samples or your SD is too high, sth like that?
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Old 05-01-2011, 06:32 PM
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Default Re: Help needed with HL 60

hI
My problem is that am losing my cells when i aspirate the medium after centrifuging my plates and thus my MTT data isnt that good.
Am asking a way how to avoid the centrifugation step but still get the MTT data taking in consideration that i have to remove the old medium + MTT

Thanks
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