Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture > Apoptosis, Autophagy, and Necrosis Forum
Register Search Today's Posts Mark Forums Read

Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


What downregulation of the genes TP53 and CASP3 stands for???

What downregulation of the genes TP53 and CASP3 stands for??? - Apoptosis, Autophagy, and Necrosis Forum

What downregulation of the genes TP53 and CASP3 stands for??? - Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-18-2010, 07:17 PM
Volunteer
Points: 2,285, Level: 30 Points: 2,285, Level: 30 Points: 2,285, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2009
Posts: 32
Thanks: 0
Thanked 0 Times in 0 Posts
Default What downregulation of the genes TP53 and CASP3 stands for???



Hello!

I am investigating apoptosis by applying qRT-PCR technique.
I have done qRT-PCR and gotten downregulation on mRNA level of TP53 and CASP3 genes in the tissue exposed to UVR. Downregulation follows by the upregulations of the genes!
I am wondering what the mechanisms behind the genes' downregulation can be in this case?!

Thank you!
Reply With Quote
  #2  
Old 10-19-2010, 02:33 PM
Warthaug's Avatar
Post-Doc
Points: 2,383, Level: 31 Points: 2,383, Level: 31 Points: 2,383, Level: 31
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2009
Location: London, Canada
Posts: 203
Thanks: 1
Thanked 68 Times in 61 Posts
Default Re: What downregulation of the genes TP53 and CASP3 stands for???

If I had to guess, UV is damaging the RNA, and it is being degraded as a consequence. UV damage also increases expression of these genes, leading to the upregulation you are observing later on.

Have you compared this to an RNA whose levels should be consistent? If I am correct, you should see down-regulation of most/all RNAs in the cell. If I'm wrong than I have no idea why you are seeing what you are seeing.

Sorry I couldn't be of more help.

Bryan
Reply With Quote
  #3  
Old 10-20-2010, 03:04 PM
Volunteer
Points: 2,285, Level: 30 Points: 2,285, Level: 30 Points: 2,285, Level: 30
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Apr 2009
Posts: 32
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: What downregulation of the genes TP53 and CASP3 stands for???

Thank you, Bryan, for your reply!

I did expose rat lenses to in vivo UVR-B, then ran evolution study with the latest latency of 5 days. Lenses cDNAs (exposed and control) were amplified with relative qRT-PCT using p53-, caspase-3 and gadd45 primers. I used 18s as a reference gene.

18s cDNA input was indifferent between exposed and control lenses likewise for gadd45 in all time intervals. p53 mRNA upregulation precedes caspase3 upregulation in the rat lens after in vivo exposure to UVR. Both TP53 and CASP3 genes are downregulated in early latencies.
It might be so that UV degrades mRNA but not really 18s rRNA as I see. I found also in P.C.Hanawalt papers that recovery of mRNA synthesis following by UVR and transcription-coupled repair (TCR) take places in DNA damaged tissues, so that perhaps I do have degradation of mRNA in earlier latencies followed by recovery of mRNA synthesis and upregulation of mRNAs.
But then, I do not observe differences in all time intervals for gadd45. Do you have any idea why?? It's mRNAs should degrade also like p53 etc. Perhaps, gadd45 upregulation on mRNA level should come later, after 5 days..

Thank you!!!
Reply With Quote
Reply

Tags
casp3 , downregulation , genes , stands , tp53


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
What downregulation of the gene stands for??? Kobrus General Science Questions and Layperson Board 0 10-17-2010 04:54 PM


All times are GMT. The time now is 09:46 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12278 seconds with 16 queries