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#1
06-25-2010, 04:50 PM
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 comet assay from frozen tissue? I need to prepare comet assay from frozen cardiac muscle tissue. Is it possible to get some cells from non-cryopreserved tissue in order to perform comet assay? I`ve tried simple homogenization and collagenase digestion methods- no positive results i.e. no cells on slides. Any solution? Help, please...  |
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#2
03-15-2011, 09:39 PM
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| Re: comet assay from frozen tissue? Quote:
You have to put fresh samples in the mincing solution, dissociate them and then you can freeze the sample for later analysis. |
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| assay , comet , frozen , tissue |
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