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Fluorescent LC3 detection

Fluorescent LC3 detection - Apoptosis, Autophagy, and Necrosis Forum

Fluorescent LC3 detection - Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


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Old 01-26-2010, 03:13 PM
Pipette Filler
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Talking Fluorescent LC3 detection



Hi

I am setting up an assay to detect LC3 expression in macrophages undergoing autophagy. Papers that I have read to date all seem to transiently transfect the cells with a GFP-LC3 construct prior to autophagy induction and then analyse by microscopy.

Has anyone out there used a fluorescent antibody to LC3 rather than doing a transfection? I thought that perhaps I could induce autophagy then add a fluorescent tagged LC3 antibody and then look by fluorescent microscopy (or confocal).

Thanks in advance
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autophagy , detection , fluorescent , fluorescent antibody , lc3


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