Fluorescent LC3 detection
I am setting up an assay to detect LC3 expression in macrophages undergoing autophagy. Papers that I have read to date all seem to transiently transfect the cells with a GFP-LC3 construct prior to autophagy induction and then analyse by microscopy.
Has anyone out there used a fluorescent antibody to LC3 rather than doing a transfection? I thought that perhaps I could induce autophagy then add a fluorescent tagged LC3 antibody and then look by fluorescent microscopy (or confocal).
Thanks in advance :)
|All times are GMT. The time now is 12:12 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved