Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Apoptosis, Autophagy, and Necrosis Forum (http://www.molecularstation.com/forum/apoptosis-autophagy-necrosis-forum/)
-   -   Fluorescent LC3 detection (http://www.molecularstation.com/forum/apoptosis-autophagy-necrosis-forum/72311-fluorescent-lc3-detection.html)

okso 01-26-2010 03:13 PM

Fluorescent LC3 detection
 
Hi

I am setting up an assay to detect LC3 expression in macrophages undergoing autophagy. Papers that I have read to date all seem to transiently transfect the cells with a GFP-LC3 construct prior to autophagy induction and then analyse by microscopy.

Has anyone out there used a fluorescent antibody to LC3 rather than doing a transfection? I thought that perhaps I could induce autophagy then add a fluorescent tagged LC3 antibody and then look by fluorescent microscopy (or confocal).

Thanks in advance :)


All times are GMT. The time now is 04:04 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08856 seconds with 11 queries