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| Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death. |
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02-12-2009, 11:50 PM
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 High Through Annexin V Assay We are looking to measure apoptosis via annexin v staining and would like to utilize a 96 well plate format. Can anyone recomend a reliable assay for this? Thanks.  |
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06-22-2009, 12:39 AM
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| Re: High Through Annexin V Assay Cellomics will give you both quantitative and qualitative data. I recommend you use PI (if you use anexinV-FITC) or BOBO (if you use annexin-alexa568) to discriminate apoptosis/necrosis. Also you should use Hoechst or DAPI for counterstaining. You are looking at apoptosis in adherent cultures. Dying cells might detach from the substrate which leads to underestimation of apoptotic events. You might want to use a collagen gel that trap detaching cells. |
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06-22-2009, 12:40 AM
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| Re: High Through Annexin V Assay Cellomics will give you both quantitative and qualitative data. I recommend you use PI (if you use anexinV-FITC) or BOBO (if you use annexin-alexa568) to discriminate apoptosis/necrosis. Also you should use Hoechst or DAPI for counterstaining. You are looking at apoptosis in adherent cultures. Dying cells might detach from the substrate which leads to underestimation of apoptotic events. You might want to use a collagen gel that trap detaching cells. |
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| annexin , assay , high |
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