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04-16-2008, 05:50 PM
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 Oligomycin B Hello everybody, I have a question about ATP depletion. I have seeded my tumor cell line on glas coverslips and treated them with oligomycin B (50µM, 4h). Before I have started my experiment I removed the medium with glucose and replaced it with glucose-free medium. After the incubation time and before fixation I wanted to wash my cells mit PBS. Unfortunately I washed all cells away. I could fix just a few cells. Okay, then I reduced the concentration 5µM for an hour. The same result. Who knows a concentration of oligomycin b for MCF-7? It is very urgent. I am grateful for all comments. Thanks in advance. Tiffy        |
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05-30-2008, 04:49 PM
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| Re: Oligomycin B Try the following web site: nature.com/onc/journal/v24/n31/fig_tab/1208667ft.html. Test 3 shows a trial using 25 pg/ml oligomycin B following several pretreatments of R6G, rotenone, and antimycin A. |
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