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| Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death. |
easy & quick assay for siRNA treated cell line samples
Apoptosis, Autophagy, and Necrosis Forum
Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.
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03-28-2008, 04:20 AM
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 easy & quick assay for siRNA treated cell line samples hi, I am a little pressed for time and wish to perform an apoptosis assay on my samples which are 'HEY cells treated with a gene-specific siRNA'.. thus looking for apoptotic changes when the gene is downregulated. Now, after a lot of compare-n-contrast between assays... I was thinking to start off by a straight western blot against PARP.. and if see some difference from untreated cells(or GFP siRNA treated HEY cells), i shall reprobe against Caspase 3 or mayb cyclins? Is my thinking ok? or shd i consider annexin or tunel or elisa? Plz suggest given the fact that i m very new to lab research. and any special tips for western... Any help will be highly appreciated. Thanks,        |
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