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Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


How to measure apoptosis / necrosis in a cell culture system?

How to measure apoptosis / necrosis in a cell culture system? - Apoptosis, Autophagy, and Necrosis Forum

How to measure apoptosis / necrosis in a cell culture system? - Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.


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Old 10-24-2008, 04:11 PM
Pipette Filler
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Default How to measure apoptosis / necrosis in a cell culture system?



I'm trying to think of the simpliest way - perhaps some sort of assay from the supernatant? Any help would be much appreciated!
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Old 10-28-2008, 03:28 PM
Pipette Filler
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Default Re: How to measure apoptosis / necrosis in a cell culture system?

You cannot characterize a particular kind of cell death with a single marker. The discrimination between necrosis and apoptosis is very dificult in vitro particularly through supernatant assay because in vitro apoptotic cells undergo a secondary necrosis.
I can advise you to consult this review to make it clearer:
Krysko & al., 2007 - Apoptosis and necrosis: Detection, discrimination and phagocytosis. Methods 44, 205-221
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