About MCF-7 cells

[prasannakhere]

Hi all/ friends, Im prasanna, doing research.Actually i'm doing research on MCF-7 cells.
My work is not at all proceeding forward due to these cells.
My first assay is trypan blue assay.
When i seed the cells into the flask they are growing well, but taking time.
But, when i trypsinise the cells for plating into 12 well plate, after 24 hrs when i observe the cells under microscope, in the middle of the wells i found group of floating cells every time. this is confusing me a lot for the further drug addition. For counting also these cells are not coming from the well.Like this i found five-six times. I myself felt a lot with this expt.This time due to the above problem i changed the medium into the wells after 24hrs, suspecting the death of cells may be due to trypsinisation. But after addition of fresh medium also, i found floating cells in the wells. Please kindly suggest some method to avoid this problem. I'm in a great trouble with this. still i was in the first assay. Is there any other method for trypsinising the cells? Or the problem is with my handling? I don't understand.Are there anybody doing work on especially MCF-7 cells? I will be very thankful to you if i solve this problem by your suggestion at the most earliest possible time.
With regards,
Prasanna lakshmi,
prasannakhere@yahoo.com

[aynagizem]

hello;
i am working with them also. which medium do you use? and do you homogenize with pipeting after adding tryipsine and incubating them in the incubator? if you dont do please try maybe this point is the problem.

best wishes
Gizem AYNA