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Apoptosis, Autophagy, and Necrosis Forum Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.

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BD Biosciences Apoptosis Kits

Apoptosis, Autophagy, and Necrosis Forum

Discuss and post questions about Apoptosis, Programmed Cell Death, Necrosis, Autophagy, and other forms of cell death.



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Old 06-29-2007, 08:36 PM
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Question BD Biosciences Apoptosis Kits

Hello everyone,
I am having problems using the BD Biosciences apoptosis kit.

Last week I got quite strange results.

It looks as if I have 2 populations in the UR- window. I contacted our technician and he thought that the compensation is wrong. But today I checked the whole configuration and everything seems to be ok, when I use annexin v or 7 AAD alone. But together it looks strange. I have attached you some pictures. HepG2 cells were treated with different oxysterols.

Help please
cheers
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