| | Need some advice
I am looking to design an antibody. I also designed the peptide sequence based on several prediction methods. However, when I submitted the sequence, I got a reply back from the company making the antibody that since my residue has a central cysteine, they cannot synthesize the antibody. However, on my antigenecity plots the peptide sequence containing this cysteine comes out as the best. On the hydrophobicity plots too, this region is the least hydrophobic. Now the question I have is: Is the presence of a single cysteine enough to make the peptide antigenic? I actually came across a paper wherein a peptide containing an internal cysteine was actually being tried as a vaccine. Can you please give me your views on this?
Thanks and hoping to hear from some of you.