Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Antibody Forum (http://www.molecularstation.com/forum/antibody-forum/)
-   -   Lowest volume in Micropipette is .2 ul, I wish to perform the following calculation (http://www.molecularstation.com/forum/antibody-forum/87970-lowest-volume-micropipette-2-ul-i-wish-perform-following-calculation.html)

Riku01 07-02-2013 04:59 AM

Lowest volume in Micropipette is .2 ul, I wish to perform the following calculation
 
I am new to working in the lab and can use some help.

Now I have a falcon tube that hold 500 ul of blocking solution and I wish to add antibody to that. I don't know how much but I do know I must perform a 1:5000 dilution. So I did the calculation I arrived to .1 ul (500/5000), that would be how many antibody I would add to 500 ul of blocking solution. However the micropipette's lowest value is .2 ul. So what do I do now? Is a simple way to obtain what I need?

Riku01 07-02-2013 05:07 AM

Re: Lowest volume in Micropipette is .2 ul, I wish to perform the following calculati
 
Ok I should rephrase that. I have falcon tube contain blocking solution and I will take out 500 ul and put it in another falcon tube or centrifuge tube whatever. And to that I will take a certain amount of antibody and add it to that amount. I calculated .1 because I need to do 1 1/5000 dilution so 500/5000 = .1, so .1 ul of antibody + 500 ul of blocking solution however the micropipette does not go to that amount so what should I do?


All times are GMT. The time now is 11:20 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08964 seconds with 11 queries