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Biotin quantitation in labeled antibodies

Biotin quantitation in labeled antibodies - Antibody Forum

Biotin quantitation in labeled antibodies - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 11-04-2011, 06:03 PM
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Question Biotin quantitation in labeled antibodies



HI all
I am using Pierce Biotin quantitation kit. Some how their positive control- Biotinylated HRP showed the correct value on day 1, the day I prepared the solution. I also aliquoted 120 ul and stored at -20, as per the manual. But the next day, positive control would not give correct values. So does anyone know if Bio-HRP loses its biotin moity on freezing and thawing. or the positive control supplied to me is bad? Please help.
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