| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-02-2011, 07:31 PM
| |||||||||||
| |||||||||||
 How to affinity purify Ab Hi, We generated rabbit sera against GST fusion proteins. And then affinity purify with the same fusion protein. There are many annoying bands on western that cross react with the purified Ab in addition to the endogenous protein (Serum preabsorbed with GST, and then binds with GST-fusion protein, and finally elute with glycine from column). Should I try like making His-tagged fusion protein and expressed in different E. coli, and affinity purify antibody that generated with GST-fusion protein? Thanks for your helps. GSH   |
|
| Tags |
| affinity , purify |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Anti-HA affinity Matrix from Roche | siba84 | Immunoprecipitation Forum | 0 | 03-31-2011 10:12 PM |
| Affinity purification of antibody against MBP-fusion | John Leonard | Protocols and Methods Forum | 0 | 06-04-2008 09:31 PM |
| Proteins Digest, Vol 22, Issue 15 | Dan Guire | Protein Forum | 0 | 03-31-2007 10:13 PM |
| RE: Re: affinity columns (Allison) (DK) | Dan Guire | Protein Forum | 0 | 03-30-2007 10:35 PM |
| Re: affinity columns (Allison) | Dan Guire | Protein Forum | 2 | 03-30-2007 09:40 PM |
Linear Mode
