Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Antibody Forum
Register Search Today's Posts Mark Forums Read

Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


How to affinity purify Ab

How to affinity purify Ab - Antibody Forum

How to affinity purify Ab - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-02-2011, 07:31 PM
GSH GSH is offline
Pipette Filler
Points: 44, Level: 1 Points: 44, Level: 1 Points: 44, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default How to affinity purify Ab



Hi,

We generated rabbit sera against GST fusion proteins. And then affinity purify with the same fusion protein. There are many annoying bands on western that cross react with the purified Ab in addition to the endogenous protein (Serum preabsorbed with GST, and then binds with GST-fusion protein, and finally elute with glycine from column). Should I try like making His-tagged fusion protein and expressed in different E. coli, and affinity purify antibody that generated with GST-fusion protein?

Thanks for your helps.
GSH
Reply With Quote
Reply

Tags
affinity , purify


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Anti-HA affinity Matrix from Roche siba84 Immunoprecipitation Forum 0 03-31-2011 10:12 PM
Affinity purification of antibody against MBP-fusion John Leonard Protocols and Methods Forum 0 06-04-2008 09:31 PM
Proteins Digest, Vol 22, Issue 15 Dan Guire Protein Forum 0 03-31-2007 10:13 PM
RE: Re: affinity columns (Allison) (DK) Dan Guire Protein Forum 0 03-30-2007 10:35 PM
Re: affinity columns (Allison) Dan Guire Protein Forum 2 03-30-2007 09:40 PM


All times are GMT. The time now is 07:19 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11356 seconds with 16 queries