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AFP Antibody

AFP Antibody - Antibody Forum

AFP Antibody - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 10-14-2011, 07:18 AM
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Default AFP Antibody



Background
Alpha-fetoprotein (AFP) is a 65 kDa glycoprotein found in the serum of the mammalian fetal liver, yolk sac and GI tract. Expression of AFP in adult cells is low. However, synthesis aberrantly occurs in adult liver cancer cells. The tumor suppressor gene p53 and β-catenin are both involved in the regulation of AFP expression. In normal adult cells p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and β-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma.
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Old 10-20-2011, 09:34 AM
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Default Re: AFP Antibody

The AFPs are single-chain glycoproteins with molecular masses ranging from 66 to 72 kDa, considered tumor-associated fetal protein and classified as a member of a three-domain albuminoid gene family that currently consists of four members : albumin (ALB), vitamin-D binding protein (DBP), AFPs, and alfa-albumin (alfa-ALB). The detectability of AFPs during ontogeny precedes the detectability of ALB by a considerable period of time. Similarly to serum albumin (sALB), serum alfa-fetoproteins (sAFPs) is known to bind and transport a multitude of ligands, including bilirubin, fatty acids, retinoids, steroids, heavy metals, dyes, flavonoids, phytoestrogens, dioxins, and various organic drugs. Unlike ALB, high concentrations of hydrophobic ligands (i.e., fatty acids, estrogens) have been reported to induce an irreversible conformational change in the tertiary structure of AFPs. It would seem that shock/stress-induced conformational (variant) forms of this fetal protein influence, modify, or contribute as dual regulators of growth, capable of both enhancement and inhibition of growth, in cancer as well as fetal cells. The discovery of the growth regulatory properties of AFPs, makes becoming this fetal protein represents an resource for the armamentarium of biological therapeutics such as entire AFP molecule or as small amino acid fragments which diverse biological properties has been also elucidated. Lastly, the immunoassay methods developed for AFP peptides, that use monoclonal antibodies, who react against various AFP isoforms, present in postnatal and adult life. However, remain still uncertain whether the 70-kDa form that we presently measure is indeed the major adult form of AFPs.
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