Antibody dilution

[nawi]

Hello,
I need someone to explain me the dilutions of antibody, need to be sure..
so my starting antibody concentration is 200 ug/500ul. I should use 1:5000 dilution.. so I made 100 ul aliquots (5 ul of concentrated antibody solution in 2495 ul dH20, I got 25 aliquots and stored them on -20 C) 1:500...
so I need to make 1500 ul solution that contains my antibodies in 1:5000 dilution.
If I put a 100 ul aliquot (1:500) and add 1400 ul of solution, will it be a 1:5000 dilution?

please, I am confused with this solutions dilutions and need a resolution.
TY

[luisillo]

The initial aliquots are fine. However, if you need 1500 ul of working solution (1:5000) you should add 150 ul of 1:500 aliquot to 1350 ul of solution.

If my calculations are fine, 100 ul of 1:500 in 1400 ul would result in a 1:7500 final concentration.

[nawi]

Yes, I will have to reduce my working solution on 1(100+900 ul) ml to get 1:5000,
thank you very much, I find this forum very helpful..

[luisillo]

Solving problems is the most important goal of science. As a scientific community, this forum and its people are only following that ideal.