I need someone to explain me the dilutions of antibody, need to be sure..
so my starting antibody concentration is 200 ug/500ul. I should use 1:5000 dilution.. so I made 100 ul aliquots (5 ul of concentrated antibody solution in 2495 ul dH20, I got 25 aliquots and stored them on -20 C) 1:500...
so I need to make 1500 ul solution that contains my antibodies in 1:5000 dilution.
If I put a 100 ul aliquot (1:500) and add 1400 ul of solution, will it be a 1:5000 dilution?
please, I am confused with this solutions dilutions and need a resolution.