I'm naive in Ig purification.
In a couple of mounths I'm going to purify mouse IgM for mouse hybridoma supernatant and then use the isolated IgM to treat rats.
I'll have a large amount of medium (at least 1lt) which purify IgM from.
The hybrydoma will be cultured in serum free / chemical defined medium, thus I know the only Ig present in the medium will be the IgM.
A collegue suggests me to use a commercial kit not specific for IgM isolation and check the different elution rate.
I have found some IgM isolation kits based on Protein L or Mannan binding protein or thiophilic matrices.
I really appreciate any suggetsion /advice you can give me.