Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

[darmst2]

Hi,

Does anyone know if there's a commercial antibody that will recognize a mouse variable light (kappa) chain?

I have a scFv that I need to detect and it doesn't contain a Fc or Constant domain so I need something specific for the Variable region.

I'm hoping there's a commercial antibody or a lab that has prepared some anti-variable antiserum that would be willing to share an aliquot???


Thanks,
Dustin

[Flatliner]

Did you find anything? I am currently in the exact same situation. Any help would be great!

[darmst2]

No, I still haven't found one...

[SarahG]

The only one I could find is from US Biologicals.

See:
1degreebio.org/product.php?pid=562424&qid=17692

From their description: "...Protein L will also bind Single Chain Variable Fragments (Sc Fv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse."

Hope this helps,
Sarah

[Flatliner]

Thanks, but I've already tried that. pL does not bind the scFv i'm trying to detect.

[Flatliner]

Originally Posted by darmst2

No, I still haven't found one...

Have you by any chance tested 1904-66N from us biological (usbio.net)?
It's a polyclonal serum raised against full length IgG's. the guys at usbio said they expect it to bind both constant and variable, but they didn't know.

[obama]

you can contact customer care of any standard antibody supplier they can help you out.

[RMcDuffie]

I've ordered custom monoclonal and polyclonal projects from Abpro aganst very diffucult targets (small peptides, high homology, GPCR, phospho-specific, cell surface proteins, ect) with very good results. If you can't find the antibody in a commercial catalogue, they can make it for you