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Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help! - Antibody Forum

Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help! - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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  #1  
Old 07-21-2010, 11:55 AM
Pipette Filler
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Default Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!



Hi,

Does anyone know if there's a commercial antibody that will recognize a mouse variable light (kappa) chain?

I have a scFv that I need to detect and it doesn't contain a Fc or Constant domain so I need something specific for the Variable region.

I'm hoping there's a commercial antibody or a lab that has prepared some anti-variable antiserum that would be willing to share an aliquot???


Thanks,
Dustin
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Old 08-30-2011, 12:52 PM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

Did you find anything? I am currently in the exact same situation. Any help would be great!
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Old 08-31-2011, 01:47 PM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

No, I still haven't found one...
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Old 09-02-2011, 06:38 PM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

The only one I could find is from US Biologicals.

See:
1degreebio.org/product.php?pid=562424&qid=17692

From their description: "...Protein L will also bind Single Chain Variable Fragments (Sc Fv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse."

Hope this helps,
Sarah
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Old 09-09-2011, 09:24 AM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

Thanks, but I've already tried that. pL does not bind the scFv i'm trying to detect.

Last edited by Flatliner; 09-09-2011 at 09:25 AM.
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Old 09-09-2011, 10:07 AM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

Quote:
Originally Posted by darmst2 View Post
No, I still haven't found one...
Have you by any chance tested 1904-66N from us biological (usbio.net)?
It's a polyclonal serum raised against full length IgG's. the guys at usbio said they expect it to bind both constant and variable, but they didn't know.
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Old 09-09-2011, 11:09 AM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

you can contact customer care of any standard antibody supplier they can help you out.
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Old 03-21-2012, 01:14 PM
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Default Re: Looking for anti-variable light chain (kappa) antibody [mouse]- Please Help!

I've ordered custom monoclonal and polyclonal projects from Abpro aganst very diffucult targets (small peptides, high homology, GPCR, phospho-specific, cell surface proteins, ect) with very good results. If you can't find the antibody in a commercial catalogue, they can make it for you
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antibody , antivariable , chain , kappa , light , mouse , scfv , variable


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