I'm new to immunohistochemistry so I hope someone can answer my question.
I need to do some IHC with mouse brain tissues. But the commercial primary antibody I need to use is only available from mouse.
Although I've seen some papers that use mouse antibody on mouse brains, I am still wondering how much noise I would get? And does anyone know if there's any trick to prepare the mouse tissue in a way to lower non-specific antibody binding when using an antibody from mouse?
Thank you very much for your help!