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can I use mouse antibody to do immunohistochemistry in mouse brain sections?

can I use mouse antibody to do immunohistochemistry in mouse brain sections? - Antibody Forum

can I use mouse antibody to do immunohistochemistry in mouse brain sections? - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 02-06-2010, 06:02 AM
Pipette Filler
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Default can I use mouse antibody to do immunohistochemistry in mouse brain sections?



Hi everyone,

I'm new to immunohistochemistry so I hope someone can answer my question.

I need to do some IHC with mouse brain tissues. But the commercial primary antibody I need to use is only available from mouse.

Although I've seen some papers that use mouse antibody on mouse brains, I am still wondering how much noise I would get? And does anyone know if there's any trick to prepare the mouse tissue in a way to lower non-specific antibody binding when using an antibody from mouse?

Thank you very much for your help!
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Old 03-12-2013, 09:02 PM
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Default Re: can I use mouse antibody to do immunohistochemistry in mouse brain sections?

It is posible to use a mouse monoclonal on a mouse specimen; however, first do a throrough antibody search. Increase your primary antibody search to obtain a primary in another specie i.e check databases like Biocompare, Antibody Directory, Linscott Directory, Abnova, Abcam, LifeTechnologies etc. If none available, you then must preabsorb any endogenous mouse IgG with a mouse IgG normal serum. Jackson Immuno has a great selection of F(ab) fragments against endogenous IgG from various species for this purpose. Following blocking step, block endogenous mouse IgG with the F(ab) fragment sera. This should block all endogenous IgG binding sites. Jackson Immuno website can elaborate on the binding mechanism of F(ab) fragments. This will help you understand the principle behind antibody-antigen binding sites.

goodluck,
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