Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Antibody Forum
Register Search Today's Posts Mark Forums Read

Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


Applications for antibodies

Applications for antibodies - Antibody Forum

Applications for antibodies - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-09-2009, 02:26 AM
Pipette Filler
Points: 29, Level: 1 Points: 29, Level: 1 Points: 29, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2009
Posts: 8
Thanks: 1
Thanked 0 Times in 0 Posts
Default Applications for antibodies



Hello everyone, I am looking for primary antibodies for immunohistochemistry on PFA-perfused, cryoprotected frozen sections.
A fair few of the antibodies list different applications like Western Blotting, ELISAs, Immunoprecipitation and not Immunohistochemistry.

I feel really confused and out of my league, and do not understand why some of these antibodies cannot be used for an immunohistochemistry and immunocytochemistry? Are they conjugated to something else?!

Thank you for helping me clarify this....
Reply With Quote
  #2  
Old 02-14-2010, 11:30 AM
Zagami's Avatar
Ph.D Doctorate
Points: 4,203, Level: 43 Points: 4,203, Level: 43 Points: 4,203, Level: 43
Activity: 99% Activity: 99% Activity: 99%
 
Join Date: Sep 2009
Location: Tabaka Mission Hospital - Kiisi -Kenya & Italy Sicily
Posts: 163
Thanks: 2
Thanked 28 Times in 24 Posts
Default Re: Applications for antibodies

Otherwise from ELISA and WB methods, immunohistochemistry (IHC) it has the ability to show where certain proteins are located, inside a tissue section (normal or in relationship to its pathology), that deriving from a specific patient (age, sex, etc.), and effected biopsy at unfixed time. In IHC procedure, tissue section must be fixed before to prevent chemical or biological changes site of located proteins. Fixation process involves that proteins and other cellular components of the tissue, cannot stir anymore, to modify conformationally of it, to interact the one of it with the others, and this condition been termed “native molecules”. The produced antibodies against native molecules, in one conformational natural state (folded state), recognize only epitopes that has been present in this molecular form, while if the same proteins has been submitted to denaturation to get a linear molecules, such as WB during the preparation gel electrophoresis, the elicites antibodies cannot be able to recognize the same epitopes expressed on the native protein, still more if these are found in the fold (epitopic inaccessibility). Nevertheless, possibility that slightly denaturing tissue can to determine changes of native molecules with epitopes expression which are able to react with the antibody that detect same antigen in ELISA and WB methods. In add, two types of monospecific first antibodies to used in immunoassay : a) clonal, and b) monoclonal. The first recognize solely very specifically selected linear epitope on the antigen molecule after its detailed proteomic analysis, whereas the monoclonal antibodies recognize very often steric epitopes that frequently change their conformation during tissue preparation (denaturation tissue, protein extraction, protein transfer in the case of western blot – related procedures, or as a consequence of the protein-protein interactions, etc.) making the corresponding monoclonal antibody less specific and less avid. The various methods, ELISA, WB and IHC, seeking the same molecule, they use antibodies that not individualize the same epitope on molecule target, for different state (native or modified) of the inducer molecular antigen at correspondent antibody formation.
Reply With Quote
  #3  
Old 09-26-2012, 05:50 AM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2012
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Applications for antibodies

Hello,

Antibodies are substances found in the blood that help the body fight infection. They recognize foreign invaders and bind to chemicals on their surfaces called antigens. The process tags bacteria, viruses, infected cells or other foreign matter for destruction by other parts of the immune system. White blood cells called B-lymphocytes produce specific antibodies for every foreign antigen the body encounters. Antibodies are also referred to as immunoglobulins and are found in the blood. They are produced by white blood cells called B cells. Antibodies are one main defense of the immune system; they identify and sometimes deactivate bacteria and viral cells.

ELISA, or enzyme-linked immunosorbent assay, is used as a diagnostic tool in medicine and in biological research to quantify the amount of a specific protein. ELISA uses antibodies to detect the protein of interest and a reagent that produces a color. The amount of color relates to the amount of protein. Antibodies are part of the body defense against foreign proteins present on a bacteria or virus for instance. An antibody is specific for a single protein; this protein target is known as an antigen. For Immunohistochemistry, it is a powerful tool for diagnosing cancer and other diseases.

Best Regards,
Stephen Jones
Reply With Quote
Reply

Tags
antibodies , applications


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Applications of the recombinant DNA Stephany Molecular Biology Techniques 0 05-23-2008 03:42 PM
Gene Therapy-System and applications aftabac Conferences , Symposiums and Meetings 0 04-30-2008 06:01 PM
Future Applications of Peptides in Research admin Peptide Forum 2 03-03-2008 08:33 PM
NIDDK Request for Applications on Genetic Studies of=20 Karp, Robert C Elegans Forum 0 11-22-2004 01:51 PM
Applications of Eigenvectors!? Steven O. Chemistry Forum 11 12-25-2003 09:02 AM


All times are GMT. The time now is 08:45 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13141 seconds with 16 queries