Precipitated Antibody Problems

[Biomencer]

Hey there Folks,

I spent a good deal of time purifying some antibodies from serum. They were kept at ~1.5mg/ml in PBS and kept at 4C. I tested them with western and they seemed to work fine. However when I went to do experiments with them the following week, I found that they were not working as well as I expected.

I noticed that the protein has precipitated, and I was wondering if there was a way of getting them back into solution without damaging the antibody.

Thank for your help. This is my first post to this forum.

~

[Kai31410]

Production of a "Blocking Type" Antibody in Rabbits
Robert Feinberg
Abstract

1. Nonprecipitating antibody of the blocking type has been produced in rabbits by multiple subcutaneous injections of antigen suspended in Freund's adjuvant.
2. Injection of animals intravenously and intramuscularly with antigen adsorbed onto alum failed to produce this type of antibody.
3. The nonprecipitating antibody detected by combination with radioiodinated antigen was precipitable by sheep antirabbit globulin and electrophoretically is a {gamma}-globulin.