Hey there Folks,
I spent a good deal of time purifying some antibodies from serum. They were kept at ~1.5mg/ml in PBS and kept at 4C. I tested them with western and they seemed to work fine. However when I went to do experiments with them the following week, I found that they were not working as well as I expected.
I noticed that the protein has precipitated, and I was wondering if there was a way of getting them back into solution without damaging the antibody.
Thank for your help. This is my first post to this forum.