Antibodies for ELISA

molecule2005 · #1 (**permalink**) 10-19-2006, 10:47 PM

What antibodies are used in ELISA - monoclonal or polyclonal?

are some specific for ELISA use?

thanks

MicroKiller · #2 (**permalink**) 01-29-2007, 09:55 PM

Antibodies being either monoclonal or polyclonal can be used for elisa. The question would be which one could give you a better sensitivity and lower background. You have to decide on which antibody to use based on what you are trying to achieve in your ELISA assay. Monoclones and polyclones each have their own advantages in elisa..

Monoclones are suppose to be of a single clone origin meaning only one similar antibody is present. This may increase specificity of your assay but in turn sacrifice your sensitivity. THis is becuase polyclonal originating from several cell lines would have antibodies in its pool that is specific to several epitopes.. therefore increasing avidity. So you may have higher sensitivity but lower specificity.. i hope this helps explain things to you..

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Antibodies for ELISA

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