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how to reduce IgG non specific binding?

how to reduce IgG non specific binding? - Antibody Forum

how to reduce IgG non specific binding? - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 11-29-2008, 12:32 AM
Pipette Filler
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Smile how to reduce IgG non specific binding?



Hi everyone, just want to ask how to reduce IgG non specific binding in my western blotting? I have specific antibodies against TRPC6 and Caveolin, but in my IgG control, I could still see their pull downs. Anyone can help me to optimize it?


Thanks a lot!
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Old 11-29-2008, 01:17 AM
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Default Re: how to reduce IgG non specific binding?

Hello,
welcome to the forums.

Would be a good idea to decrease non-specific binding by better blocking, or using blocking in more steps ie Primary antibody incubation in addition to blocking steps.

Try 2-5% blotto milk, or other methods of blocking to see which one is best.

let us know what you find
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Old 12-02-2008, 09:42 AM
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Default Re: how to reduce IgG non specific binding?

My lab uses block ace from AbD Serotec. [Only registered users see links. ] . It has given us good consistent results
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