how to reduce IgG non specific binding?

[dizue]

Hi everyone, just want to ask how to reduce IgG non specific binding in my western blotting? I have specific antibodies against TRPC6 and Caveolin, but in my IgG control, I could still see their pull downs. Anyone can help me to optimize it?


Thanks a lot!

[admin]

Hello,
welcome to the forums.

Would be a good idea to decrease non-specific binding by better blocking, or using blocking in more steps ie Primary antibody incubation in addition to blocking steps.

Try 2-5% blotto milk, or other methods of blocking to see which one is best.

let us know what you find

[mphl]

My lab uses block ace from AbD Serotec. [Only registered users see links. ] . It has given us good consistent results