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Method to Concentrate Tissue Culture Supernatant for Antibody?

Method to Concentrate Tissue Culture Supernatant for Antibody? - Antibody Forum

Method to Concentrate Tissue Culture Supernatant for Antibody? - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 03-05-2008, 07:31 AM
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Question Method to Concentrate Tissue Culture Supernatant for Antibody?



Hi,
does anyone have the best method to concentrate tissue culture supernatants for antibody?

Im trying to concentrate 1 to 5 liters of hybridoma supernatants with murine IgG.

What are others are using to concentrate these supernatants for protein A or G column purification?

thanks!
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Old 04-09-2008, 02:30 PM
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Default Re: Method to Concentrate Tissue Culture Supernatant for Antibody?

Hello Dionisia,
the best way to do this is either salt or chemical precipitation, centrifugation, or concentration method such as lyophilization.

what are you trying currently?

Zonal or gradient centrifugation is probably best depending on the size/nature of your protein.
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Old 03-15-2009, 09:01 AM
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Default Re: Method to Concentrate Tissue Culture Supernatant for Antibody?

probably u need a couple of steps from separation to polishing... cause with 1 to 5 litres.. would be best to bring the volume down first.. concentrate the supernatant first to bring it to a more manageable volume.. then u can do protein a,g, size exclusion, anionic exchange etc...
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