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| Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries. |
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02-26-2008, 08:21 AM
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 Polyclonal Antibodies Generation from Recombinant Protein Purification Hello guys, I am trying to produce polyclonal antibodies. I am using Ecoli and trying to purify my recombinant protein from inclusion bodies. Currently i am looking for the best method to purify my protein from the inclusion bodies samples to inject into rabbit. Has anyone done this that can help me?  I have HIS-tagged my recombinant proteins which I produced in E.coli. Should I solubilize the pellet using my prepared solubilization buffer (8M urea, 100mM NaH2PO4, 10mM Tris). However I am not sure whether urea will affect the rabbit polyclonal generation? Thanks I really appreciate any help.   |
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03-20-2008, 06:43 AM
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 Re: Polyclonal Antibodies Generation from Recombinant Protein Purification Using the SDS-PAGE, Urea will run out from the gels. Otherwise, you can dialyze your protein before injection into rabbit |
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03-15-2009, 10:03 AM
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| Re: Polyclonal Antibodies Generation from Recombinant Protein Purification i agree with izacil.. buffer exchange before injecting!!! |
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| Tags |
| antibodies , generation , polyclonal , protein , purification , recombinant |
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