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02-26-2008, 07:21 AM
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 Polyclonal Antibodies Generation from Recombinant Protein Purification Hello guys, I am trying to produce polyclonal antibodies. I am using Ecoli and trying to purify my recombinant protein from inclusion bodies. Currently i am looking for the best method to purify my protein from the inclusion bodies samples to inject into rabbit. Has anyone done this that can help me?  I have HIS-tagged my recombinant proteins which I produced in E.coli. Should I solubilize the pellet using my prepared solubilization buffer (8M urea, 100mM NaH2PO4, 10mM Tris). However I am not sure whether urea will affect the rabbit polyclonal generation? Thanks I really appreciate any help.         |
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Re: Polyclonal Antibodies Generation from Recombinant Protein Purification 
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