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Polyclonal Antibodies Generation from Recombinant Protein Purification

Polyclonal Antibodies Generation from Recombinant Protein Purification - Antibody Forum

Polyclonal Antibodies Generation from Recombinant Protein Purification - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 02-26-2008, 08:21 AM
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Question Polyclonal Antibodies Generation from Recombinant Protein Purification



Hello guys,
I am trying to produce polyclonal antibodies. I am using Ecoli and trying to purify my recombinant protein from inclusion bodies.

Currently i am looking for the best method to purify my protein from the inclusion bodies samples to inject into rabbit.

Has anyone done this that can help me?

I have HIS-tagged my recombinant proteins which I produced in E.coli.

Should I solubilize the pellet using my prepared solubilization buffer (8M urea, 100mM NaH2PO4, 10mM Tris). However I am not sure whether urea will affect the rabbit polyclonal generation?

Thanks I really appreciate any help.
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Old 03-20-2008, 05:43 AM
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Default Re: Polyclonal Antibodies Generation from Recombinant Protein Purification

Using the SDS-PAGE, Urea will run out from the gels.
Otherwise, you can dialyze your protein before injection into rabbit
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Old 03-15-2009, 09:03 AM
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Default Re: Polyclonal Antibodies Generation from Recombinant Protein Purification

i agree with izacil.. buffer exchange before injecting!!!
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antibodies , generation , polyclonal , protein , purification , recombinant


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