I am trying to produce polyclonal antibodies. I am using Ecoli and trying to purify my recombinant protein from inclusion bodies.
Currently i am looking for the best method to purify my protein from the inclusion bodies samples to inject into rabbit.
Has anyone done this that can help me?
I have HIS-tagged my recombinant proteins which I produced in E.coli.
Should I solubilize the pellet using my prepared solubilization buffer (8M urea, 100mM NaH2PO4, 10mM Tris). However I am not sure whether urea will affect the rabbit polyclonal generation?
Thanks I really appreciate any help.