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| Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries. |
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| Hello, I have had a synthetic antibody generated for me that is supposed to bind to a specific protein of interest expressed in the pancreas. I have done a Western blot using rabbit sera to blot a membrane containing islet cell extracts. I chose rabbits that did not have any noise in the area of my protein of interest and am having them injected with my synthetic antibody. When I collect my synthetic antibody from these rabbits I want to confirm the antibody is binding to my protein of interest so I will do a Western and look for a band in the area of my protein of interest (this band should not have been present in the first Western). I don't think this will directly confirm that my antibody is binding to my protein of interest. QUESTION: HOW WOULD I DIRECTLY CONFIRM MY ANTIBODY IS BINDING TO MY PROTEIN OF INTEREST? Thanks very much!!! |
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| Wow thanks for the quick and very helpful reply!!! ![]() So I would have to run two gels: 1 would be used for blotting with antibody 2 would be used for punching out my positive western band Is this correct or am I misinterpreting something? Thanks very much again!! |
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| antibody , confirming , specificity |
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