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Covalent labeling of antibody on solid phase

Covalent labeling of antibody on solid phase - Antibody Forum

Covalent labeling of antibody on solid phase - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 09-25-2007, 12:12 PM
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Default Covalent labeling of antibody on solid phase



Hi there,

I would like to covalently label an antibody with Alexa 647 following the protocol described in E. Lundberg et al. (J Immunol Methods. 322 (2007) 40-49) but I am not sure about the use of the TopTip Protein G micropipette tips (Glygen Corp.). In the paper it is mentioned that one micropipette tip contains 30 -50 Āµg of protein A according to the manufacturer. However, I can't find any information as to how much protein G is contained in the corresponding tips containing protein G. I need this piece of information to calculate the amount of dye needed. Has anyone already tried this method of antibody labeling? I would appreciate any piece of advice. Thanks.

Greetings

Pheebz
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antibody , covalent , labeling , phase , solid


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