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Guanidine HCL and Anti-albumin depletion kit

Guanidine HCL and Anti-albumin depletion kit - Antibody Forum

Guanidine HCL and Anti-albumin depletion kit - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 09-21-2007, 07:26 PM
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Default Guanidine HCL and Anti-albumin depletion kit



According to this article, [Only registered users see links. ] , Guanidine HCL really disrupts the antigen binding site of the antibody and even the Fc terminus that binds to protein A (all of this at concentrations too low to create noticeable structural conformational disruptions).

Does anyone know of an elution buffer (Mass Spec compatible) that will allow my albumin depletion kit to keep hold of albumin and keep it bound while consistently eluting albumin attached peptides?

Normally I would use cibachron blue to capture albumin, but am now looking specifically for an antibody capture of albumin.
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Old 09-27-2007, 04:28 PM
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Default Re: Guanidine HCL and Anti-albumin depletion kit

Update---too unlikely to find such a gentle elution buffer. Anyone with similar problem can note that I solved this issue by using a MW cutoff filter column to separate albumin from smaller proteins/peptides after elution with the "harsh" elution buffer.
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antialbumin , depletion , guanidine , hcl , kit


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