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Purification of Antibodies and Removal of DNA
I have to isolate antibodies away from contaminating DNA from a large scale monoclonal antibody preparations (starting volume before purification ~100L).
I would like to do this using filtration at some point if possible.
Anyone have suggestions?
Filters? Chromotography? Other methods to use?
Re: Purification of Antibodies and Removal of DNA
You can use protein A/G to capture your antibodies. Then wash extensively with a wash buffer, followed by elution by a high salt buffer.
I am not sure how to handle 100L of starting volume though.
|antibodies , dna , purification , removal|
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