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| Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries. |
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#1
08-27-2007, 01:45 PM
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 Purification of Antibodies and Removal of DNA Hey, I have to isolate antibodies away from contaminating DNA from a large scale monoclonal antibody preparations (starting volume before purification ~100L). I would like to do this using filtration at some point if possible. Anyone have suggestions? Filters? Chromotography? Other methods to use?  |
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#2
08-27-2007, 04:17 PM
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| Re: Purification of Antibodies and Removal of DNA You can use protein A/G to capture your antibodies. Then wash extensively with a wash buffer, followed by elution by a high salt buffer. I am not sure how to handle 100L of starting volume though. |
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| antibodies , dna , purification , removal |
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