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Purification of Antibodies and Removal of DNA

Purification of Antibodies and Removal of DNA - Antibody Forum

Purification of Antibodies and Removal of DNA - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


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Old 08-27-2007, 01:45 PM
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Post Purification of Antibodies and Removal of DNA



Hey,
I have to isolate antibodies away from contaminating DNA from a large scale monoclonal antibody preparations (starting volume before purification ~100L).

I would like to do this using filtration at some point if possible.


Anyone have suggestions?

Filters? Chromotography? Other methods to use?
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Old 08-27-2007, 04:17 PM
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Default Re: Purification of Antibodies and Removal of DNA

You can use protein A/G to capture your antibodies. Then wash extensively with a wash buffer, followed by elution by a high salt buffer.

I am not sure how to handle 100L of starting volume though.
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