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Difference in custom antibody recognition

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Old 07-10-2007, 05:15 PM
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Default Difference in custom antibody recognition

Hello:
We have an issue with the antibody generated against custom synthesized peptide. We have got good results with immunocytochemistry experiments, where as under SDS-PAGE it doesn't recognize the right molecular size as it's supposed to. Can anyone explain this? I dont know where is the problem lies?
Thank you
Anil
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