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Primary antibody species
I am going to be doing immunohistochemistry on rabbit tissue. Is it ok if I use a primary rabbit polyclonal antibody. Or do I have to use an antibody which is raised in another species, but can react with rabbit?
I always get confused about this. Hope to recieve comments,
Re: Primary antibody species
If you're probing rabbit, you need to use a primary that was not raised in rabbit.
The reason for this is because your secondary (if it is against rabbit) will be staining your tissue directly, in the absence of your primary antibody.
You could test this by going through your protocol but omitting the primary antibody. If you get staining from your secondary antibody alone, you can't use that secondary.
|antibody , primary , species|
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