Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > Antibody Forum
Register Search Today's Posts Mark Forums Read

Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


Primary antibody species

Primary antibody species - Antibody Forum

Primary antibody species - Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-28-2007, 12:15 PM
Pipette Filler
Points: 1,299, Level: 20 Points: 1,299, Level: 20 Points: 1,299, Level: 20
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2007
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Question Primary antibody species



Hello,
I am going to be doing immunohistochemistry on rabbit tissue. Is it ok if I use a primary rabbit polyclonal antibody. Or do I have to use an antibody which is raised in another species, but can react with rabbit?
I always get confused about this. Hope to recieve comments,
MS.
Reply With Quote
  #2  
Old 06-28-2007, 01:42 PM
Graduate Student
Points: 2,923, Level: 35 Points: 2,923, Level: 35 Points: 2,923, Level: 35
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2006
Location: Ithaca, NY
Posts: 145
Thanks: 0
Thanked 17 Times in 12 Posts
Default Re: Primary antibody species

If you're probing rabbit, you need to use a primary that was not raised in rabbit.

The reason for this is because your secondary (if it is against rabbit) will be staining your tissue directly, in the absence of your primary antibody.

You could test this by going through your protocol but omitting the primary antibody. If you get staining from your secondary antibody alone, you can't use that secondary.
Reply With Quote
Reply

Tags
antibody , primary , species


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Natural selection is proven wrong gim Biology Forum 3 05-15-2009 07:43 PM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
primary vs secondary antibody public_enemee Western Blot Forum 1 10-31-2008 06:01 AM
Western background staining with Primary antibody (Rabbit) Sharon Waldrop Protocols and Methods Forum 2 08-19-2008 01:44 PM
[Arabidopsis] gene names in different species? Nan Eckardt Arabidopsis and Plant Biology 0 10-26-2007 09:49 PM


All times are GMT. The time now is 02:23 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11993 seconds with 16 queries