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| Antibody Forum Antibody Forum. Ask and discuss antibody suppliers, antibody related techniques and protocols, and antibody production such as using phage display libraries. |
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05-22-2007, 06:59 PM
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 Protein Determination before WB Is it sugested that when one separates a peptide and stores it at -80C in separate aliquots and then takes out an aliquot. Should a repeat BCA protein assay be run before a new WB is run?        |
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06-12-2007, 10:35 PM
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| Re: Protein Determination before WB It shouldn't hurt, although you don't have to; I think it depends on what you use the peptide for. I would certainly run a BCA or Bradford to quantify the total protein in your sample that you actually run in the Western blot. However, I have used aliqots of a peptide that I quantified once and never again, and I've had no problems. |
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