I have been using many commercially available ELISA kits for my research. Now I am trying to develop an ELISA assay using an antibody I have against chondroitin sulfate found in blood. Any kind soul want to give me a hand in this with regard to
How do I determinte the antibody titer? Checker board? Any elaboration on that?
How do I coat plate with the primary antibody?
Where do I buy the labelled secondary from?
How do I create the standard curve?
Any help,directions will be greatly appreciate. Thanks in advance.