Uptake of bare Morpholinos by pancreata of rat pups
Previous work by K. Prasadan showed Morpholinos enter rodent embryonic pancreatic cells from the extracellular space. Several papers from University Paris Diderot-CNRS show uptake into the pancreas of rat pups after s.c. injection of bare or fluor-tagged Morpholinos:
Uzan B, Figeac F, Portha B, Movassat J. [Only registered users see links. ] PLoS ONE. 2009;4(3):e4734. Epub 2009 Mar 6.
Figeac F, Uzan B, Faro M, Chelali N, Portha B, Movassat J. [Only registered users see links. ] Am J Physiol Endocrinol Metab. 2009 Nov 17. [Epub ahead of print]
While we at Gene Tools are habitually skeptical of claims of delivery of bare Morpholinos dosed systemically in vivo, this is a case where previous careful work has shown uptake of bare oligos into embryonic cells of this type. We anticipate further work in this system with keen interest.
__________________ Jon D. Moulton, Ph.D.
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