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issues with making a gateway compatible luciferase expression vector

issues with making a gateway compatible luciferase expression vector - Animal and Molecular Model Systems

issues with making a gateway compatible luciferase expression vector - Discuss and Post Questions about Animal Model Systems such as Mouse, Rat, Pig, Sheep etc.


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Old 10-16-2009, 02:27 PM
Pipette Filler
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Default issues with making a gateway compatible luciferase expression vector



Hi,
I am trying to insert the gateway cassette (according to Invitrogen instructions from their kit) into pGL3basic to receive a gateway compatible luciferase reporter vector that is promoterless. Unfortunately, I cannot get any colonies ever. I am pretty frustrated and under dealine pressure... any suggestions? HEEEEEEEEEEELP!
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compatible , expression , gateway , gatewayadapted , issues , luciferase , making , promoterless , vector


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