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| Hi, I am trying to insert the gateway cassette (according to Invitrogen instructions from their kit) into pGL3basic to receive a gateway compatible luciferase reporter vector that is promoterless. Unfortunately, I cannot get any colonies ever. I am pretty frustrated and under dealine pressure... any suggestions? HEEEEEEEEEEELP! |
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| compatible , expression , gateway , gatewayadapted , issues , luciferase , making , promoterless , vector |
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