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-   -   issues with making a gateway compatible luciferase expression vector (http://www.molecularstation.com/forum/animal-molecular-model-systems/70970-issues-making-gateway-compatible-luciferase-expression-vector.html)

lab_tech 10-16-2009 02:27 PM

issues with making a gateway compatible luciferase expression vector
 
Hi,
I am trying to insert the gateway cassette (according to Invitrogen instructions from their kit) into pGL3basic to receive a gateway compatible luciferase reporter vector that is promoterless. Unfortunately, I cannot get any colonies ever. I am pretty frustrated and under dealine pressure... any suggestions? HEEEEEEEEEEELP!


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