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-   -   Effect of different agarose concentrations (http://www.molecularstation.com/forum/agarose-gel-electrophoresis-forum/87999-effect-different-agarose-concentrations.html)

wigald 07-13-2013 10:23 AM

Effect of different agarose concentrations
 
Hi folks,

Could somebody plz help me by explaining the effect of different agarose concentrations in an agarose gel?

Will the resolution or the band separation improved by higher concentrations?

Thx in advance

luisillo 07-15-2013 02:21 PM

Re: Effect of different agarose concentrations
 
The higher the concentration of agarose in a gel (for DNA electrophoresis), the less the migration of the DNA bands but the better the resolution of them (DNA bands).

In other words, it is the resolution what gets improved by higher concentrations, while lower concentration favour faster migration.

wigald 07-23-2013 06:05 PM

Re: Effect of different agarose concentrations
 
Thank you!

Zagami 07-24-2013 09:44 AM

Re: Effect of different agarose concentrations
 
thinks also to electroendosmosis of agarose powdre!


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