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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Bands not defined

Bands not defined - Agarose Gel Electrophoresis Forum

Bands not defined - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 06-04-2013, 07:01 PM
Pipette Filler
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Default Bands not defined



Hi, I've been trying to run gels for a series of PCRs to test primers. However the bands so far have been curvy, slanted and very unclear. I then tested a 100bp and 1kb marker in a gel I made to check that the error is from the gel electrophoresis and not the PCR. A similar problem occured however, and I can't seem to figure out what is wrong.

I'll attach both pictures. The first gel was run at 80V and is a 3% gel. The second gel is also 3% and it was run at 90V.

If anyone has any ideas on what to fix that would be great.

Thanks!
Attached Images
File Type: jpg Gel 1.jpg (17.9 KB, 1 views)
File Type: jpg Gel 2.jpg (16.9 KB, 1 views)
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Old 06-04-2013, 09:35 PM
Pipette Filler
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Default Re: Bands not defined

Here is another picture of a gel I did to give another example of what I was trying to explain.
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File Type: jpg Gel 3.jpg (26.0 KB, 1 views)
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