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Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Tips for RFLP gel

Tips for RFLP gel - Agarose Gel Electrophoresis Forum

Tips for RFLP gel - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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  #1  
Old 04-22-2013, 03:11 PM
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Talking Tips for RFLP gel



Hi,

I am doing some PCR-RFLP (Restriction Fragment Length Polymorphism) and running samples on a Polyacrilamyde gel (6%) and silver staining to visualize DNA fragments.

The issue is: Some of my restriction enzymes need BSA to work, the problem is that it also stains and appear as a thick band above the DNA fragments of interest. Although this does not interfere in the visualization it does not let me take a clear and big picture as I have to cut it to avoid picturing BSA band.

I send the pic. I am asking for suggestions on how to get rid or at least take out of the picture the thick BSA band. By the way, in the pic I attached the above band is BSA and the below bands are DNA fragments.

Thank you.
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Old 06-18-2013, 02:48 PM
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Talking Re: Tips for RFLP gel

I don't often post a thread at molecular station, but when I do...

only a 30% is replied by other people.

xD
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Old 06-19-2013, 03:41 PM
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Talking Re: Tips for RFLP gel

Quote:
Originally Posted by luisillo View Post
only a 30% is replied by other people.
Sorry, this part shoud say: there is less than a 30% chance for it to be replied by other people.

It is fun for me xD
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