Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Agarose Gel Electrophoresis Forum
Register Search Today's Posts Mark Forums Read

Agarose Gel Electrophoresis Forum Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


DNA band has a smear after gel electrophoresis run

DNA band has a smear after gel electrophoresis run - Agarose Gel Electrophoresis Forum

DNA band has a smear after gel electrophoresis run - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 06-14-2012, 08:45 AM
Pipette Filler
Points: 3,634, Level: 39 Points: 3,634, Level: 39 Points: 3,634, Level: 39
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Oct 2007
Posts: 19
Thanks: 0
Thanked 1 Time in 1 Post
Default DNA band has a smear after gel electrophoresis run



Hi!
could you please help me, I have a smear after gel electrophoresis run in my Plasmid band.
Thanks
Reply With Quote
  #2  
Old 06-15-2012, 02:11 AM
butters's Avatar
M.D/Ph.D
Points: 4,938, Level: 47 Points: 4,938, Level: 47 Points: 4,938, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Location: Malaysia
Posts: 315
Thanks: 25
Thanked 68 Times in 63 Posts
Default Re: DNA band has a smear after gel electrophoresis run

Miss-pcr, you have missed few detail information.

1. What is the size of your plasmid?
2. What is the percent of your gel run?
3. Did you include DNA ladder if yes is the separation good?
4. Picture please.
5. What extraction method did you use?
6. Do you re-use your buffer / gel if yes too many times?
7. Is this the first run or twice with identical result?
8. Have your labmate having problem with their electrophoresis too?

sometime in questions we find answers.
Reply With Quote
  #3  
Old 01-11-2013, 07:48 PM
Graduate Student
Points: 247, Level: 4 Points: 247, Level: 4 Points: 247, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 100
Thanks: 0
Thanked 0 Times in 0 Posts
Default バーバリー 時計

[

[

[
Reply With Quote
Reply

Tags
band , dna , electrophoresis , gel , run , smear


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Agarose gel electrophoresis of PCR products - Method and Protocol domba PCR - Polymerase Chain Reaction Forum 12 02-24-2012 03:39 AM
AFLP smear Yuma248 Protocols and Methods Forum 0 08-13-2009 10:12 AM
TBE vs TAE for Gel Electrophoresis dnamolecule Basic Lab Protocols and Techniques 5 11-15-2007 06:27 AM
RNA electrophoresis admin Article Discussion 0 03-17-2007 04:44 AM
Electrophoresis Buffers - Unlimited Lifespan? Wolfgang Schechinger Protocols and Methods Forum 2 12-28-2005 09:59 AM


All times are GMT. The time now is 02:44 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12446 seconds with 16 queries