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Genomic DNA in Agarose Gel
I would like to know whether I can run a ~50Kb Genomic DNA using agarose gel? Its just one single type of genomic DNA and I would like to check the quality of the DNA (to see what percentage of DNA is of complete length). Can someone suggest the methods I should use for this kind of experiment?
What agarose % is good? Some references I came across suggest 0.3% agarose gel but that should be very fragile from my understanding.
Does the gel size matter? I have prepared mini-gels for analyzing some very short PCR fragments before but have no idea about genomic DNA of ~50Kb size.
I am new to this field and trying to learn some things. May be someone here can help me with suggestions from your experience.
Have a good day!!!
Re: Genomic DNA in Agarose Gel
Even with a 03.% gel, I doubt that the 50kb band will migrate far in the gel.
|agarose , agarose gel , dna , gel , genomic , genomic dna|
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