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Plasmid isolation problem(s)

Plasmid isolation problem(s) - Agarose Gel Electrophoresis Forum

Plasmid isolation problem(s) - Agarose Gel Electrophoresis Forum. A forum to discuss electrophoresis staining, troubleshooting, and development of films for DNA and RNA gels.


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Old 12-17-2011, 10:52 AM
Pipette Filler
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Default Plasmid isolation problem(s)



Hello,

I really need your help with this!
At lab work we wanted to isolate one plasmid and later get rid of RNA.
First we have a control with no RNase, second with a small amount of RNase, third with bigger amount of it. Then another control for LiCl, a sample with LiCl added, sixth was done with PEG and last one with CTAB.

Can anyone help me and tell me what went wrong?

Thank you!
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