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-   -   Reducing bubbles when setting agarose gel (http://www.molecularstation.com/forum/agarose-gel-electrophoresis-forum/83327-reducing-bubbles-when-setting-agarose-gel.html)

oblongata 08-11-2011 03:38 PM

Reducing bubbles when setting agarose gel
 
Recently I came across a problem when preparing the agarose gel.

Specifics: After boiling the agarose mixture (agarose + TBE) via microwave, I observed that there were many microbubbles in the gel. I decided to do the gel anyway, the end result was poor migration of DNA bands, specifically that parts of the DNA bands seem to lag behind considerably and looked similar to using a toothpick to drag a blotch of spilled ink around.

Any ideas? (I will upload a picture as soon as I get to work)

JennYeap 08-13-2011 06:05 AM

Re: Reducing bubbles when setting agarose gel
 
normally after pouring the heated agarose mixture onto gel cast, u can use a pipette tip to push microbubbles to corner of the gel. this can prevent bubbles obstructing the migration of you DNA. good luck.

SAFIA 08-13-2011 01:23 PM

Re: Reducing bubbles when setting agarose gel
 
dear
i suggest you to reduce the boiling time in microwave
if you are heating the agarose for 2 min. in microwave reduce the time to one minute if solution is not clear then additional half min. should be given
n also u can remove bubbles with pipette tips

oblongata 08-14-2011 09:07 AM

Re: Reducing bubbles when setting agarose gel
 
thanks for the replies guys. It turns out that the agarose was not completely dissolved in the solution.

admin 08-14-2011 10:22 AM

Re: Reducing bubbles when setting agarose gel
 
Great tips above, I would not over heat it as well - take breaks during microwaving as it can get super hot and bubble

moh_eg 01-11-2012 11:47 AM

Re: Reducing bubbles when setting agarose gel
 
1- dont let it cool below 50C-
2- then add Et. Bromide and swirl the flask on the Bench


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